多重ゲノム工学

多重ゲノム工学的手法および加速進化による細胞のプログラミング: Multiplex Automated Genome Engineering (MAGE)

この手法Multiplex Automated Genome Engineering (MAGE)を使えば、定期的に（数時間単位）細菌のゲノムに複数のDNA配列が自動挿入され、様々な種類のゲノム改変細菌を合成できます。

..."multiplex automated genome engineering (MAGE) for large-scale programming and evolution of cells. MAGE simultaneously targets many locations on the chromosome for modification in a single cell or across a population of cells, thus producing combinatorial genomic diversity."
... "We applied MAGE to optimize the 1-deoxy-d-xylulose-5-phosphate (DXP) biosynthesis pathway in Escherichia coli"... "Twenty-four genetic components in the DXP pathway were modified simultaneously using a complex pool of synthetic DNA, creating over 4.3 billion combinatorial genomic variants per day."
..."Our multiplex approach embraces engineering in the context of evolution by expediting the design and evolution of organisms with new and improved properties."

(Programming cells by multiplex genome engineering and accelerated evolution. Harris H. Wang. et al. Nature 460, 894-898 (2009)より)

関連：Gibson Assembly Song：MAGEを使ったiGEMプロジェクト歌にしたもの